Acetylcholinesterase inhibitive activity-guided isolation of two new alkaloids from seeds of Peganum nigellastrum Bunge by an in vitro TLC- bioautographic assay

@article{Zheng2009AcetylcholinesteraseIA,
  title={Acetylcholinesterase inhibitive activity-guided isolation of two new alkaloids from seeds of Peganum nigellastrum Bunge by an in vitro TLC- bioautographic assay},
  author={Xiyu Zheng and Zijia Zhang and Guixin Chou and Tao Wu and Xue Cheng and Changhong Wang and Zheng-Tao Wang},
  journal={Archives of Pharmacal Research},
  year={2009},
  volume={32},
  pages={1245-1251}
}
Acetylcholinesterase inhibitors (AChEIs) currently form the basis of the newest drugs available for the treatment of Alzheimer’s disease. For the aim of screening effective AChEIs, the methanol extracts of the seeds of genus Peganum were found to show significant inhibitory activity of acetylcholinesterase enzyme (AChE) using an in vitro TLC-bioautographic assay. In further studies to seed of P. nigellastrum Bunge, activity-guided fractionation led to the isolation of two new alkaloids… 

Antitumor quinazoline alkaloids from the seeds of Peganum harmala

A phytochemical study on the methanol extracts from the seeds of Peganum harmala L. led to a new quizonaline alkaloid (S)-vasicinone-1-O-β-d-glucopyranoside, which screened for antiproliferative activity against human gastric cancer cells MCG-803 with MTT method.

Identification of Acetylcholinesterase Inhibitors from Seeds of Plants of Genus Peganum by Thin-Layer Chromatography-Bioautography

The TLC fingerprints combined with the bioautographic method could distinguish the seeds of the different species of genus Peganum investigated, and harmine and harmaline displayed similar AChE inhibition compared to galanthamine.

Screening of Medicinal Plants from Iranian Traditional Medicine for Acetylcholinesterase Inhibition

As the amounts of harmaline and harmine in the extracts were correlated with the IC50 values of the extracts, it can be concluded that these two alkaloids are responsible for the AChE inhibitory activity of P. harmala.

Alkaloids of Peganum harmala L. and their Pharmacological Activity

Peganum harmala L. contains 17 alkaloids of quinazoline and indole structure types. Of these, harmaline, harmine, harmalol and L-peganin (vazicin) are pharmacologically active. It was established

Peganumine B-I and two enantiomers: new alkaloids from the seeds of Peganum harmala Linn. and their potential cytotoxicity and cholinesterase inhibitory activities

Ten new alkaloids (peganumine B-I and two enantiomers), containing five β-carbolines, three quinazolones, two compounds with both of the above skeletons, and one amphoteric alkaloid with a

Discovery of Helminthosporin, an Anthraquinone Isolated from Rumex abyssinicus Jacq as a Dual Cholinesterase Inhibitor

Helminthosporin has been identified as a brain permeable dual cholinesterase inhibitor, and thus its further synthetic exploration is warranted for optimization of its potency.

Acetylcholinesterase inhibition effects of marine fungi

Results indicates that some ethyl acetate extracts of marine fungi isolated from Lianyungang area of China could inhibit AChE potently, therefore, some novel A cholinesterase inhibitors might exist in those extracts.

Cytotoxicity of alkaloids isolated from Peganum harmala seeds.

Harmine was the most potent in inhibiting cell growth, and vasicinone was most active as antiproliferating substance, and the TAF had significant cytotoxic as well as antipoliferating activity in several tumor cell lines.
...

References

SHOWING 1-10 OF 28 REFERENCES

Study of the in vitro antimicrobial activity of harmine, harmaline and their derivatives.

Antitumour principles from Peganum harmala seeds.

Results obtained indicate that alkaloids of Peganum have a high cell toxicity in vitro, and possess significant antitumour potential, which could prove useful as a novel anticancer therapy.

Activities of extract and constituents of Banisteriopsis caapi relevant to parkinsonism

Pharmacological evaluation of novel Alzheimer's disease therapeutics: acetylcholinesterase inhibitors related to galanthamine.

Because of its composite profile, including duration of action, oral therapeutic index and pharmacokinetics, P11149 is considered the better therapeutic candidate for the treatment of Alzheimer's disease.

Inhibition of Monoamine Oxidase A by β-Carboline Derivatives

Harmine, 2-methylharminium, 2,9-dimethylharminum, and harmaline were the most effective inhibitors of the purified MAO A, with lowKivalues of 5, 69, 15, and 48 nM, respectively.

Beta-carbolines, psychoactive compounds in the mammalian body. Part I: Occurrence, origin and metabolism.

We review the occurrence in nature and the formation and biotransformation in mammals of beta-carbolines, the condensation products of tryptophan and indolealkylamines with aldehydes, with special

In vitro cell-toxicity of Peganum harmala alkaloids on cancerous cell-lines.

A rapid TLC bioautographic method for the detection of acetylcholinesterase and butyrylcholinesterase inhibitors in plants.

A simple and rapid bioautographic enzyme assay on TLC plates has been developed for the screening of acetylcholinesterase and butyrylcholinesterase inhibition by plant extracts. Enzyme activity was

Analysis of harmine and harmaline of Peganum harmala in different parts and different localities

OBJECTIVE The methodology of quantitative analysis of harmine and harmaline in Peganum harmala was investigated and the contents of harmine and harmaline in Peganum harmala in different parts and