Acetylcholinesterase inhibitive activity-guided isolation of two new alkaloids from seeds of Peganum nigellastrum Bunge by an in vitro TLC- bioautographic assay

  title={Acetylcholinesterase inhibitive activity-guided isolation of two new alkaloids from seeds of Peganum nigellastrum Bunge by an in vitro TLC- bioautographic assay},
  author={Xiyu Zheng and Zijia Zhang and Guixin Chou and Tao Wu and Xue Cheng and Changhong Wang and Zheng-Tao Wang},
  journal={Archives of Pharmacal Research},
Acetylcholinesterase inhibitors (AChEIs) currently form the basis of the newest drugs available for the treatment of Alzheimer’s disease. For the aim of screening effective AChEIs, the methanol extracts of the seeds of genus Peganum were found to show significant inhibitory activity of acetylcholinesterase enzyme (AChE) using an in vitro TLC-bioautographic assay. In further studies to seed of P. nigellastrum Bunge, activity-guided fractionation led to the isolation of two new alkaloids… 

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The TLC fingerprints combined with the bioautographic method could distinguish the seeds of the different species of genus Peganum investigated, and harmine and harmaline displayed similar AChE inhibition compared to galanthamine.

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Results indicates that some ethyl acetate extracts of marine fungi isolated from Lianyungang area of China could inhibit AChE potently, therefore, some novel A cholinesterase inhibitors might exist in those extracts.

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Harmine was the most potent in inhibiting cell growth, and vasicinone was most active as antiproliferating substance, and the TAF had significant cytotoxic as well as antipoliferating activity in several tumor cell lines.



Study of the in vitro antimicrobial activity of harmine, harmaline and their derivatives.

Antitumour principles from Peganum harmala seeds.

Results obtained indicate that alkaloids of Peganum have a high cell toxicity in vitro, and possess significant antitumour potential, which could prove useful as a novel anticancer therapy.

Activities of extract and constituents of Banisteriopsis caapi relevant to parkinsonism

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Beta-carbolines, psychoactive compounds in the mammalian body. Part I: Occurrence, origin and metabolism.

We review the occurrence in nature and the formation and biotransformation in mammals of beta-carbolines, the condensation products of tryptophan and indolealkylamines with aldehydes, with special

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A rapid TLC bioautographic method for the detection of acetylcholinesterase and butyrylcholinesterase inhibitors in plants.

A simple and rapid bioautographic enzyme assay on TLC plates has been developed for the screening of acetylcholinesterase and butyrylcholinesterase inhibition by plant extracts. Enzyme activity was

Analysis of harmine and harmaline of Peganum harmala in different parts and different localities

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