Acetylation and alternative splicing regulate ZNF76-mediated transcription.

  title={Acetylation and alternative splicing regulate ZNF76-mediated transcription.},
  author={G. Zheng and Yu-Chung Yang},
  journal={Biochemical and biophysical research communications},
  volume={339 4},
  • G. Zheng, Yu-Chung Yang
  • Published 2006
  • Biology, Medicine
  • Biochemical and biophysical research communications
We previously showed that ZNF76 is a general transcription repressor targeting TATA-binding protein (TBP), through a process regulated by sumoylation [G. Zheng, Y.C. Yang, ZNF76, a novel transcriptional repressor targeting TATA-binding protein, is modulated by sumoylation, J. Biol. Chem. 279 (2004) 42410-42421]. In this study, two additional regulatory mechanisms for ZNF76 were identified. ZNF76 is acetylated by p300 and deacetylated by HDAC1, and acetylation of ZNF76 leads to its loss of… Expand
Transcription factor abundance controlled by an auto-regulatory mechanism involving a transcription start site switch
This work reveals a new negative auto-regulatory mechanism involving an alternative transcription start site (TSS) usage and uncovered that the TSS switch that mediates this regulation implies the differential expression of two transcripts with an opposite protein production ability due to their different 5′ untranslated regions. Expand
Adhesion-dependent Skp2 transcription requires selenocysteine tRNA gene transcription-activating factor (STAF).
It is demonstrated that STAF is essential and sufficient for Skp2 promoter activity and plays a role in the adhesion-dependent expression of Skp 2 and ultimately cell proliferation. Expand
The transcriptional repressor ZBP-89 and the lack of Sp1/Sp3, c-Jun and Stat3 are important for the down-regulation of the vimentin gene during C2C12 myogenesis.
  • M. Salmon, Z. Zehner
  • Biology, Medicine
  • Differentiation; research in biological diversity
  • 2009
From these studies, it is concluded that the combinatorial effect of the down-regulation of positive-acting transcription factors such as Sp1/Sp3, c-Jun and Stat3 versus the up- regulation of the repressor ZBP-89 contributes to the "turning off" of the vimentin gene during myogenesis. Expand
Tip60 modulates PLAGL2‐mediated transactivation by acetylation
This is the first demonstration that Tip60 can function as a sumoylation inhibitor in part through its intrinsic acetyltransferase activity to regulate specific gene expression. Expand
ZFP148 (Zinc-Finger Protein 148) Binds Cooperatively With NF-1 (Nuclear Factor 1) to Inhibit Smooth Muscle Marker Gene Expression During Abdominal Aortic Aneurysm Formation
ZFP148 plays a critical role in multiple murine models of aneurysm formation and elimination of NF-1 using shRNA approaches suggests that ZFP148 is important in the regulation of proliferation, smooth muscle gene downregulation, and apoptosis in aneurYSm development. Expand
Znf76 is associated with development of the eyes, midbrain, MHB, and hindbrain in zebrafish embryos
Data indicate that Znf76 governs developmental processes in the MHB, hindbrain, and eyes in zebrafish embryos, and the Fgf8 signaling networks associated with the ZnF76 function are discussed. Expand
Expression of human papillomavirus type 16 E7 is sufficient to significantly increase expression of angiogenic factors but is not sufficient to induce endothelial cell migration.
The results suggest that the mechanism of HPV 16 E7-mediated increased levels of VEGF is pRb-independent, and the individual contribution of E6 and E7 to angiogenesis was investigated. Expand
This chapter discusses the research methodology and results, as well as the aims and objectives of the study, and some of the methods used to achieve these goals. Expand


ZNF76, a Novel Transcriptional Repressor Targeting TATA-binding Protein, Is Modulated by Sumoylation*
The results suggest that ZNF76 functions as a transcriptional repressor through its interaction with TBP and that sumoylation modulates its transcriptional repression activity. Expand
ZNF76 and ZNF143 Are Two Human Homologs of the Transcriptional Activator Staf*
Findings demonstrate that ZNF76 and ZNF143 are two members of a same family of transactivator proteins that are 64 and 84% identical to their Xenopus counterpart, respectively. Expand
Site-specific Acetylation of the Fetal Globin Activator NF-E4 Prevents Its Ubiquitination and Regulates Its Interaction with the Histone Deacetylase, HDAC1*
It is shown that NF-E4 interacts directly with, and serves as a substrate for, the acetyltransferase co-activator PCAF, a component of a protein complex that facilitates the preferential expression of the human γ-globin genes in fetal erythroid cells. Expand
Acetylation of p53 activates transcription through recruitment of coactivators/histone acetyltransferases.
Results with acetylation-defective p53 demonstrate that the critical function of acetylations is not to increase the DNA binding affinity of p53 but rather to promote coactivator recruitment and histoneacetylation. Expand
Acetylation of p53 Inhibits Its Ubiquitination by Mdm2*
This study develops a method to obtain pure acetylated p53 proteins from cells and provides the direct evidence that acetylation of the C-terminal domain is sufficient to abrogate its ubiquitination by Mdm2, which has significant implications regarding a general mechanism by which protein acetylations modulates ubiquitinations-dependent proteasome proteolysis. Expand
Transforming Growth Factor-β Stimulates p300-dependent RUNX3 Acetylation, Which Inhibits Ubiquitination-mediated Degradation*
The Runt domain transcription factors (RUNXs) play essential roles in normal development and neoplasias. Genetic analyses of animals and humans have revealed the involvement of RUNX1 in hematopoiesisExpand
Duration of Nuclear NF-κB Action Regulated by Reversible Acetylation
It is demonstrated that the RelA subunit of NF-κB is subject to inducible acetylation and that acetylated forms of RelA interact weakly, if at all, with IκBα. Expand
Regulation of E2F1 activity by acetylation
It is suggested that acetylation stimulates the functions of the non‐RB bound ‘free’ form of E2F1, and it is found that the RB‐associated histone de acetylase can deacetylate E2f1. Expand
Activation of p53 Sequence-Specific DNA Binding by Acetylation of the p53 C-Terminal Domain
It is demonstrated that p53 can be modified by acetylated both in vivo and in vitro, indicating a novel pathway for p53 activation and providing an example of an acetylation-mediated change in the function of a nonhistone regulatory protein. Expand
Control of Smad7 stability by competition between acetylation and ubiquitination.
It is demonstrated that the acetylated residues in Smad7 also are targeted by ubiquitination and that acetylation of these lysine residues prevents subsequent ubiquitinations. Expand