Acetate is the preferred substrate for long-chain fatty acid synthesis in isolated spinach chloroplasts.

@article{Roughan1979AcetateIT,
  title={Acetate is the preferred substrate for long-chain fatty acid synthesis in isolated spinach chloroplasts.},
  author={P. G. Roughan and Ross Holland and Cathy Slack},
  journal={The Biochemical journal},
  year={1979},
  volume={184 3},
  pages={565-9}
}
1. Commercially available [2-14C]pyruvate and [2-14C]malonate were found to contain 3-6% (w/w) of [14C]acetate. 2. The contaminating [14C]acetate was efficiently utilized for fatty acid synthesis by isolated chloroplasts, whereas the parent materials were poorer substrates. 3. Maximum incorporation rates of the different substrates examined were (ng-atoms of C/h per mg of chlorophyll): [1-14C]acetate, 2676; [2-14C]pyruvate, 810; H14CO3-, 355; [2-14C]malonate, 19. 4. Products of CO2 fixation… CONTINUE READING

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