Acetate accumulation through alternative metabolic pathways in ackA − pta − poxB − triple mutant in E. coli B (BL21)

@article{Phue2010AcetateAT,
  title={Acetate accumulation through alternative metabolic pathways in ackA − pta − poxB − triple mutant in E. coli B (BL21)},
  author={Je-Nie Phue and Sang Yup Lee and Jeanne B. Kaufman and Alejandro Negrete and Joseph Shiloach},
  journal={Biotechnology Letters},
  year={2010},
  volume={32},
  pages={1897-1903}
}
Individual deletions of acs and aceA genes in E. coli B (BL21) showed little difference in the metabolite accumulation patterns but deletion of the ackA gene alone or together with pta showed acetic acid gradually accumulated to 3.1 and 1.7 g/l, respectively, with a minimal extended lag in bacterial growth and a higher pyruvate formation. Single poxB deletion in E. coli B (BL21) or additional poxB deletion in the ackA-pta mutants did not change the acetate accumulation pattern. When the acetate… CONTINUE READING

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