Accurate sampling of high-frequency motions in proteins by steady-state (15)N-{(1)H} nuclear Overhauser effect measurements in the presence of cross-correlated relaxation.

@article{Ferrage2009AccurateSO,
  title={Accurate sampling of high-frequency motions in proteins by steady-state (15)N-\{(1)H\} nuclear Overhauser effect measurements in the presence of cross-correlated relaxation.},
  author={Fabien Ferrage and David Cowburn and Ranajeet Ghose},
  journal={Journal of the American Chemical Society},
  year={2009},
  volume={131 17},
  pages={6048-9}
}
The steady-state {(1)H}-(15)N NOE experiment is used in most common NMR analyses of backbone dynamics to accurately ascertain the effects of the fast dynamic modes. We demonstrate here that, in its most common implementation, this experiment generates an incorrect steady state in the presence of CSA/dipole cross-correlated relaxation leading to large errors in the characterization of these high-frequency modes. This affects both the quantitative and qualitative interpretation of (15)N backbone… CONTINUE READING

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