Accurate quantitation of mitochondrial DNA reveals uniform levels in human blastocysts irrespective of ploidy, age, or implantation potential.

@article{Victor2017AccurateQO,
  title={Accurate quantitation of mitochondrial DNA reveals uniform levels in human blastocysts irrespective of ploidy, age, or implantation potential.},
  author={Andrea R. Victor and Alan J Brake and Jack C Tyndall and Darren K. Griffin and Christo G Zouves and Frank L Barnes and Manuel Viotti},
  journal={Fertility and sterility},
  year={2017},
  volume={107 1},
  pages={34-42.e3}
}
OBJECTIVE To accurately determine mitochondrial DNA (mtDNA) levels in human blastocysts. DESIGN Retrospective analysis. SETTING IVF clinic. PATIENT(S) A total of 1,396 embryos derived from 259 patients. INTERVENTION(S) Blastocyst-derived trophectoderm biopsies were tested by next-generation sequencing (NGS) and quantitative real-time polymerase chain reaction (qPCR). MAIN OUTCOME MEASURE(S) For each sample the mtDNA value was divided by the nuclear DNA value, and the result was… CONTINUE READING

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Mitochondrial DNA quantity as a biomarker for blastocyst implantation potential.

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