Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

@article{Vandesompele2001AccurateNO,
  title={Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes},
  author={Jo Vandesompele and Katleen De Preter and Filip Pattyn and Bruce Poppe and Nadine Van Roy and Anne de Paepe and Frank Speleman},
  journal={Genome Biology},
  year={2001},
  volume={3},
  pages={research0034.1 - research0034.11}
}
  • Jo Vandesompele, Katleen De Preter, +4 authors Frank Speleman
  • Published 2001
  • Biology, Medicine
  • Genome Biology
  • BackgroundGene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes. [...] Key Result We have evaluated ten housekeeping genes from different abundance and functional classes in various human tissues, and demonstrated that the conventional use of a single gene for normalization leads to relatively large errors in a significant proportion of…Expand Abstract

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