Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Abstract

Gene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes. Given the increased sensitivity, reproducibility and large dynamic range of this methodology, the requirements for a proper… (More)
DOI: 10.1186/gb-2002-3-7-research0034

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