Accurate multiplex gene synthesis from programmable DNA microchips

@article{Tian2004AccurateMG,
  title={Accurate multiplex gene synthesis from programmable DNA microchips},
  author={Jingdong Tian and Hui Gong and Nijing Sheng and Xiaochuan Zhou and Erdogan Gulari and Xiaolian Gao and George M. Church},
  journal={Nature},
  year={2004},
  volume={432},
  pages={1050-1054}
}
Testing the many hypotheses from genomics and systems biology experiments demands accurate and cost-effective gene and genome synthesis. Here we describe a microchip-based technology for multiplex gene synthesis. Pools of thousands of ‘construction’ oligonucleotides and tagged complementary ‘selection’ oligonucleotides are synthesized on photo-programmable microfluidic chips, released, amplified and selected by hybridization to reduce synthesis errors ninefold. A one-step polymerase assembly… Expand
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