Accurate measurements of coupling constants from two-dimensional nuclear magnetic resonance spectra of proteins and determination of phi-angles.

@article{Ludvigsen1991AccurateMO,
  title={Accurate measurements of coupling constants from two-dimensional nuclear magnetic resonance spectra of proteins and determination of phi-angles.},
  author={S. Ludvigsen and Karen Vitting Andersen and Flemming M. Poulsen},
  journal={Journal of molecular biology},
  year={1991},
  volume={217 4},
  pages={731-6}
}
A new and simple method to measure 3JHNH alpha coupling constants of proteins by adding and subtracting traces from corresponding two-dimensional nuclear Overhauser enhanced spectroscopy and two-dimensional correlated spectroscopy cross peaks after scaling is proposed. The optimal scaling for the addition and the subtraction of the two traces is obtained by minimizing an error function. The method was proven to give accurate and precise measurements of coupling constants when tested with a… CONTINUE READING

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