Accurate measurement of fast endocytic recycling kinetics in real time

@article{Jonker2020AccurateMO,
  title={Accurate measurement of fast endocytic recycling kinetics in real time},
  author={Caspar T H Jonker and C. Deo and Patrick J Zager and A. N. Tkachuk and A. M. Weinstein and E. Rodriguez-Boulan and L. Lavis and Ryan Schreiner},
  journal={Journal of Cell Science},
  year={2020},
  volume={133}
}
ABSTRACT The fast turnover of membrane components through endocytosis and recycling allows precise control of the composition of the plasma membrane. Endocytic recycling can be rapid, with some molecules returning to the plasma membrane with a half time <5 min. Existing methods to study these trafficking pathways utilize chemical, radioactive or fluorescent labeling of cell surface receptors in pulse-chase experiments, which require tedious washing steps and manual collection of samples. Here… Expand

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