Accurate in vitro transcription of Xenopus laevis mitochondrial DNA from two bidirectional promoters.

@article{Bogenhagen1986AccurateIV,
  title={Accurate in vitro transcription of Xenopus laevis mitochondrial DNA from two bidirectional promoters.},
  author={Daniel F. Bogenhagen and Barbara K Yoza},
  journal={Molecular and cellular biology},
  year={1986},
  volume={6 7},
  pages={2543-50}
}
The mitochondrial RNA polymerase from Xenopus laevis oocytes was partially purified by heparin-Sepharose chromatography and phosphocellulose chromatography. This RNA polymerase preparation specifically initiated the transcription of X. laevis mitochondrial DNA (mtDNA) from two bidirectional promoters contained within a 123-base-pair segment of the mtDNA between the heavy-strand replication origin and the rRNA cistrons. Transcription in vitro initiated from precisely the same start sites… CONTINUE READING