Accurate flow cytometric membrane potential measurement in bacteria using diethyloxacarbocyanine and a ratiometric technique.

@article{Novo1999AccurateFC,
  title={Accurate flow cytometric membrane potential measurement in bacteria using diethyloxacarbocyanine and a ratiometric technique.},
  author={D J Novo and Nancy G Perlmutter and RichardH. Hunt and Howard M. Shapiro},
  journal={Cytometry},
  year={1999},
  volume={35 1},
  pages={
          55-63
        }
}
BACKGROUND Membrane potential (MP) plays a critical role in bacterial physiology. Existing methods for MP estimation by flow cytometry are neither accurate nor precise, due in part to the heterogeneity of size of the particles analyzed. The ratio of a size- and MP-sensitive measurement, and an MP-independent, size-sensitive measurement, should provide a better estimate of MP. METHODS Flow cytometry and spectrofluorometry were used to detect red (488 --> 600 nm) fluorescence associated with… CONTINUE READING
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