Accurate determination of microbial diversity from 454 pyrosequencing data

@article{Quince2009AccurateDO,
  title={Accurate determination of microbial diversity from 454 pyrosequencing data},
  author={C. Quince and A. Lanz{\'e}n and T. Curtis and R. Davenport and N. Hall and I. Head and L. F. Read and W. Sloan},
  journal={Nature Methods},
  year={2009},
  volume={6},
  pages={639-641}
}
We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it can be accurately calculated. 
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