Accurate cleavage and polyadenylation of exogenous RNA substrate.

  title={Accurate cleavage and polyadenylation of exogenous RNA substrate.},
  author={Claire L. Moore and Phillip A. Sharp},
  volume={41 3},
Purified precursor RNA containing the L3 polyadenylation site of late adenovirus 2 mRNA is accurately cleaved and polyadenylated when incubated with nuclear extract from HeLa cells. The reaction is very efficient; 75% of the precursor is correctly processed. Cleavage is rapidly followed by polymerization of an initial poly(A) tract of approximately 130 nucleotides. Additional adenosine residues are added during further incubation. In the presence of the ATP analog alpha-beta-methylene-adenosine… CONTINUE READING


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