Accurate Strand-Specific Quantification of Viral RNA

  title={Accurate Strand-Specific Quantification of Viral RNA},
  author={Nicole E. Plaskon and Zach N Adelman and Kevin M. Myles},
  booktitle={PloS one},
The presence of full-length complements of viral genomic RNA is a hallmark of RNA virus replication within an infected cell. As such, methods for detecting and measuring specific strands of viral RNA in infected cells and tissues are important in the study of RNA viruses. Strand-specific quantitative real-time PCR (ssqPCR) assays are increasingly being used for this purpose, but the accuracy of these assays depends on the assumption that the amount of cDNA measured during the quantitative PCR… CONTINUE READING


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