Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome

@article{Shendure2005AccurateMP,
  title={Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome},
  author={Jay A. Shendure and Gregory Porreca and Nikos Basil Reppas and Xiaoxia Nina Lin and John P. McCutcheon and Abraham M. Rosenbaum and Michael D. Wang and Kun Zhang and Robi David Mitra and George M. Church},
  journal={Science},
  year={2005},
  volume={309},
  pages={1728 - 1732}
}
We describe a DNA sequencing technology in which a commonly available, inexpensive epifluorescence microscope is converted to rapid nonelectrophoretic DNA sequencing automation. We apply this technology to resequence an evolved strain of Escherichia coli at less than one error per million consensus bases. A cell-free, mate-paired library provided single DNA molecules that were amplified in parallel to 1-micrometer beads by emulsion polymerase chain reaction. Millions of beads were immobilized… Expand
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