Accurate Measurement of the Thickness of Ultrathin Sections by Interference Microscopy

In quantitative electron microscopy, a knowledge of the thickness (0) of the sections is often required . For example, when objects smaller than 0 (e .g . ribosomes) are counted in sections, 0 must be known in order that the observed numbers may be related to the unit volume of the section . Even when comparatively large structures are submitted to… CONTINUE READING