Accuracy of protein secondary structure determination from circular dichroism spectra based on immunoglobulin examples.

@article{Tetin2003AccuracyOP,
  title={Accuracy of protein secondary structure determination from circular dichroism spectra based on immunoglobulin examples.},
  author={Sergey Y Tetin and Franklyn G. Prendergast and Sergei Yu Venyaminov},
  journal={Analytical biochemistry},
  year={2003},
  volume={321 2},
  pages={183-7}
}
Strong contribution of the aromatic amino acid side chain chromophores to the far-UV circular dichroism (CD) spectra substantially distorts a relatively weak CD signal originating from beta sheet, the main type of immunoglobulin secondary structure. In this study we compared the secondary structure calculated from the far-UV CD spectra with the X-ray data for three antibody Fab fragments. Calculations were performed with three different algorithms, using two sets of reference proteins. Low… CONTINUE READING