Accuracy and reproducibility of a multiplex immunoassay platform: a validation study.

  title={Accuracy and reproducibility of a multiplex immunoassay platform: a validation study.},
  author={Julie A. Bastarache and Tatsuki Koyama and Nancy E. Wickersham and Daphne B. Mitchell and Ray L. Mernaugh and Lorraine B. Ware},
  journal={Journal of immunological methods},
  volume={367 1-2},
BACKGROUND Multiplex immunoassays offer many advantages over singleplex assays for the analysis of multiple analytes in a single sample. We sought to validate a specific multiplex cytokine immunoassay (Human 9-plex cytokine array on the Searchlight® platform by Thermoscientific) prior to use in a large clinical study. METHODS We compared spike and recovery of recombinant proteins on the Searchlight® platform to singleplex immunoassays purchased from R&D Systems, measured identical patient… CONTINUE READING


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