Accuracy, precision, and stability in measurement of hemoglobin A1C by "high-performance" cation-exchange chromatography.

@article{Schifreen1980AccuracyPA,
  title={Accuracy, precision, and stability in measurement of hemoglobin A1C by "high-performance" cation-exchange chromatography.},
  author={R S Schifreen and J M Hickingbotham and George N. Bowers},
  journal={Clinical chemistry},
  year={1980},
  volume={26 3},
  pages={466-72}
}
We present a "high-performance" liquid-chromatographic method for determination of HbA1c with use of a carboxylate cation-exchange column, ethanolic mobile phases, and a hemolysate-storage reagent that stabilizes a sample for up to five weeks at 4 degrees C. Temperature control and the addition of ethanol to the phosphate step-gradient increase precision by… CONTINUE READING