Accumulation of lipid production in Chlorella minutissima by triacylglycerol biosynthesis-related genes cloned from Saccharomyces cerevisiae and Yarrowia lipolytica

  title={Accumulation of lipid production in Chlorella minutissima by triacylglycerol biosynthesis-related genes cloned from Saccharomyces cerevisiae and Yarrowia lipolytica},
  author={Hsin-Ju Hsieh and Chia-Hung Su and Liang-Jung Chien},
  journal={Journal of Microbiology},
Discovery of an alternative fuel is now an urgent matter because of the impending issue of oil depletion. Lipids synthesized in algal cells called triacylglycerols (TAGs) are thought to be of the most value as a potential biofuel source because they can use transesterification to manufacture biodiesel. Biodiesel is deemed as a good solution to overcoming the problem of oil depletion since it is capable of providing good performance similar to that of petroleum. Expression of several genomic… Expand
Effect of Single and Combined Expression of Lysophosphatidic Acid Acyltransferase, Glycerol-3-Phosphate Acyltransferase, and Diacylglycerol Acyltransferase on Lipid Accumulation and Composition in Neochloris oleoabundans
The single and multiple expression of heterologous genes LPAT, GPAT, and DGAT showed to significantly enhanced the lipid accumulation in N. oleoabundans, showing the potential for the generation of microalgal strains with higher economical potential forThe production of lipids. Expand
Towards engineering the microalga Chlorella sorokiniana for the production of tailored high-value oils
These investigations confirmed the common finding that nitrogen stress is a robust way to induce neutral lipid accumulation, but also highlighted the potential of pH change as an alternate stressor for TAG production and developed and begins to characterise a toolbox of genetic parts for nuclear transformation. Expand
The role of diatom glucose-6-phosphate dehydrogenase on lipogenic NADPH supply in green microalgae through plastidial oxidative pentose phosphate pathway
This report represents a promising candidate to engineer lipid biosynthesis in heterologous hosts with notable commercial significance, and it highlights the potential role of plastidial PPP in supplying lipogenic NADPH in microalgae. Expand
Heterologous expression of DGAT genes in the marine microalga Tetraselmis chui leads to an increase in TAG content
Five clones showed TAG contents notably higher than the wild-type (WT) strain, achieving TAG increases from 40% up to 115% of dry weight (mg g−1), the highest reported in microalgae to date and serve as a proof of concept regarding the possibility of using this technology to markedly improve oil content in microalgalae or any other product suitable for genetically engineered enhancement. Expand
Overexpression of acetyl-CoA synthetase (ACS) enhances the biosynthesis of neutral lipids and starch in the green microalga Chlamydomonas reinhardtii
Genetic engineering can be the solution to achieve the economically feasible production of microalgal based biofuels and other bulk materials. A good number of microalgal species can growExpand
Increased fatty acid accumulation following overexpression of glycerol-3-phosphate dehydrogenase and suppression of β-oxidation in oleaginous fungus Mortierella alpina
Mortierella alpina is an industrial oleaginous fungus. Although its fatty acid synthesis pathways have been thoroughly examined, the mechanism for fatty acid accumulation is still not fullyExpand
Nitrogen Source Affects Glycolipid Production and Lipid Accumulation in the Phytopathogen Fungus Ustilago maydis
The results indicate that U. maydis accumulates lipid droplet when deprived of nitrogen source and confirm that UA is not under nitrogen control, but rather that MEL and lipid droplets are produced and oppositely regulated by nitrogen. Expand
Advances in culture and genetic modification approaches to lipid biosynthesis for biofuel production and in silico analysis of enzymatic dominions in proteins related to lipid biosynthesis in algae
Based on primary amino acid sequences and tertiary structure of proteins shared by certain algae, it is likely that these proteins may relate to lipid yield and accumulation, which makes bioinformatics a powerful tool for in silico study of proteins and for selecting genes involved in lipid biosynthesis that could be useful for heterologous transformation in algae. Expand
Enhancement of lipid accumulation in microalgae by metabolic engineering.
This review summarizes recent research about a variety of microalgal lipids biosynthesis pathways, and discusses multiple gene manipulation strategies that have been employed for specific lipid overproduction in industrial microalgae. Expand
Fatty Acids from Microalgae: Targeting the Accumulation of Triacylglycerides
Microalgae were originally considered as sources of long-chain polyunsaturated fatty acids (PUFAs), mainly for aquaculture purposes. However, based on the fact that their fatty acids (FA), stored asExpand


Involvement of the G3P shuttle and β-oxidation pathway in the control of TAG synthesis and lipid accumulation in Yarrowia lipolytica.
The findings suggest that Y. lipolytica has a modified and unique metabolism of glycerol that is dedicated to G3P synthesis (and also to TAG synthesis) which may contribute to its oleaginous character. Expand
The effect of mixotrophy on microalgal growth, lipid content, and expression levels of three pathway genes in Chlorella sorokiniana
Nannochloropsis oculata CCMP 525, Dunaliella salina FACHB 435, and Chlorella sorokiniana CCTCC M209220 were compared in mixotrophic and photoautotrophic cultures in terms of growth rate, protein, andExpand
DGA1 (diacylglycerol acyltransferase gene) overexpression and leucine biosynthesis significantly increase lipid accumulation in the Deltasnf2 disruptant of Saccharomyces cerevisiae.
We previously found that SNF2, a gene encoding a transcription factor forming part of the SWI/SNF (switching/sucrose non-fermenting) chromatin-remodelling complex, is involved in lipid accumulation,Expand
Modification of seed oil content and acyl composition in the brassicaceae by expression of a yeast sn-2 acyltransferase gene.
The yeast sn-2 acyltransferase has been shown to encode a protein that can exhibit lysophosphatidic acid acyl transferase activity and that can be used to change total fatty acid content and composition as well as to alter the stereospecific acyl distribution of fatty acids in seed TAGs. Expand
Identification and expression analysis of castor bean (Ricinus communis) genes encoding enzymes from the triacylglycerol biosynthesis pathway.
The results represent snapshots of global transcriptional activities of genes encompassing six enzyme families involved in castor bean TAG biosynthesis that are present during seed development that represent potential targets for biotechnological approaches to produce nutritionally and industrially desirable oils. Expand
Lipids from heterotrophic microbes: advances in metabolism research.
The present review aims to summarize recent details in lipid metabolism research and engineering, as well as culture condition optimization and innovations, that can all contribute to higher productivity and further advancement of the field. Expand
The Initial Step of the Glycerolipid Pathway
  • Z. Zheng, J. Zou
  • Biology, Medicine
  • The Journal of Biological Chemistry
  • 2001
The initial step of phospholipid biosynthesis in yeast is carried out through the acylation of glycerol 3-phosphate (G-3-P) and dihydroxyacetone phosphate by stereospecificsn-1 acyltransferases. HereExpand
Genetic Engineering of Algae for Enhanced Biofuel Production
Potential avenues of genetic engineering that may be undertaken in order to improve microalgae as a biofuel platform for the production of biohydrogen, starch-derived alcohols, diesel fuel surrogates, and/or alkanes are focused on. Expand
Enzymes of triacylglycerol synthesis and their regulation.
De novo biosynthesis of triacylglycerol in eukaryotic cells, the isoenzymes that are involved, their subcellular locations, how they are regulated, and their putative individual roles in glycerolipid biosynthesis are focused on. Expand
Rapid, colorimetric quantification of lipid from algal cultures.
The method allowed reliable detection and quantification of fatty acid content from 1 to 2 mL of algal culture and the total cost per assay was estimated at less than $5, representing a significant cost savings over traditional lipid quantification procedures. Expand