Accumulation of intracellular HCO3- by Na(+)-HCO3- cotransport in interlobular ducts from guinea-pig pancreas.

@article{Ishiguro1996AccumulationOI,
  title={Accumulation of intracellular HCO3- by Na(+)-HCO3- cotransport in interlobular ducts from guinea-pig pancreas.},
  author={Hiroaki Ishiguro and Martin C. Steward and Allan R. G. Lindsay and R. Maynard Case},
  journal={The Journal of physiology},
  year={1996},
  volume={495 ( Pt 1)},
  pages={169-78}
}
1. Short segments of interlobular duct were microdissected from guinea-pig pancreas following enzymatic digestion. After overnight culture, intracellular pH (pH1) and Na+ concentration ([Na+]i) were measured by microfluorometry in duct cells loaded with either the pH-sensitive fluoroprobe 2'7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) or the sodium-binding benzofuran isophthalate (SBFI). 2. The transporters responsible for maintaining pHi above equilibrium were investigated by using… CONTINUE READING

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