Accumulation of biopolymers in activated sludge biomass

  title={Accumulation of biopolymers in activated sludge biomass},
  author={Hong Chua and Peter Hoi-fu Yu and Chee Keung Louis Ma},
  journal={Applied Biochemistry and Biotechnology},
In this study, activated sludge bacteria from a conventional wastewater treatment process were induced to accumulate polyhydroxyalkanoates (PHAs) under different carbon-nitrogen (C:N) ratios. As the C:N ratio increased from 20 to 140, specific polymer yield increased to a maximum of 0.38 g of polymer/g of dry cell mass while specific growth yield decreased. The highest overall polymer production yield of 0.11 g of polymer/g of carbonaceous substrate consumed was achieved using a C:N ratio of… 
Polyhydroxyalkanoate (PHA) production in open mixed cultures using waste activated sludge as biomass
In this work, volatile fatty acids (VFAs) were used as a carbon source to assess the ability of bacteria present in waste activated sludge (WAS), as indigenous flora, to accumulate
Waste Activated Sludge as Biomass for Production of Commercial-Grade Polyhydroxyalkanoate (PHA)
PurposeThis paper presents an assessment of the production of polyhydroxyalkanoates (PHA) in biomass sourced from wastewater treatment plants (WWTPs). The purpose was to examine both the potential
Study of PHAs (polyhydroxyalkanoates) production from activated sludge.
Sludge deposition is of high concern for managers of wastewater treatment plants due to limited landfill space and increases in deposition fees. Reduction of the amount of sludge can benefit a
Production of biodegradable plastics from chemical wastewater — A novel method to reduce excess activated sludge generated from industrial wastewater treatment
Biological polymers produced by microbial fermentation are naturally biodegradable and are potential environment-friendly substitutes for synthetic plastics. However, broader applications are
Bioplastics from Waste Activated Sludge-Batch Process
Activated sludge from a full-scale pulp-paper industry wastewater treatment plant was used as a source of microorganisms to produce biodegradable plastics (polyhydroxyalkanoates) (PHA) at 25°C , pH 7
The composition analysis and preliminary cultivation optimization of a PHA-producing microbial consortium with xylose as a sole carbon source.
Though this work is at the very beginning and the PHA yield is relatively low, producing PHA from xylose by using microbial consortia is a promising way to save the P HA production cost.
Influence of the C/N ratio on the performance of polyhydroxybutyrate (PHB) producing sequencing batch reactors at short SRTs.
For the microbial selection and biomass production stage of a PHA production process carbon limitation seems favourable and nutrient deficient wastewaters may consequently require supplementation with nutrients for the selection of a stable PHA storing biomass with a high storage capacity.
Microstructure of copolymers of polyhydroxyalkanoates produced by glycogen accumulating organisms with acetate as the sole carbon source
The so-called glycogen accumulating organisms (GAOs), found in mixed culture anaerobic-aerobic wastewater treatment processes, are used to produce polyhydroxyalkanoates (PHAs) copolymers from a
The influence of process parameters on the characteristics of polyhydroxyalkanoates produced by mixed cultures.
In this study PHA produced under aerobic dynamic feeding (ADF) conditions are characterized and the introduction of a purification step during the polymer extraction process allowed the elimination of possible contaminants but did not significantly improve the polymer quality.
Lipid storage compounds in raw activated sludge microorganisms for biofuels and oleochemicals production
Activated sludge contains a microbial population responsible for the biological treatment of wastewater. This microbial population mostly consists of heterothrophic bacteria which utilize the organic


Coupling of waste water treatment with storage polymer production.
Activated sludge bacteria in a conventional waste water treatment system were induced, by controlling the carbon-nitrogen (C:N) ratio in the reactor liquor, to accumulate storage polymers, without significantly affecting the organic treatment efficiency in the waste watertreatment system.
Production of poly-3-hydroxyalkanoates from CO and H2 by a novel photosynthetic bacterium
A novel process is described to efficiently photoconvert low-grade organic materials such as waste biomass into natural biological plastics. When heterogeneous forms of dry biomass are thermally
Mole fraction control of poly(3-hydroxybutyric-co-3-hydroxyvaleric) acid in fed-batch culture of Alcaligenes eutrophus
Abstract The aims of this study were to investigate the reaction kinetics of production of poly(3-hydroxybutyric-co-3-hydroxyvaleric) acid, P(HB-co-HV), to control its mole fraction in a fermentor,
Production of polyhydroxyalkanoates by Azotobacter vinelandii UWD in beet molasses culture
Beet molasses (BM) has proven to be an excellent feedstock for polyhydroxyalkanoate (PHA) production by Azotobacter vinelandii UWD. The substrate-cost for PHA production from BM in fed-batch culture
Biosynthesis and characterization of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in Alcaligenes eutrophus.
A proposed biosynthetic pathway of P(3HB-co-4HB) film was shown to be biodegradable in an activated sludge and to have random sequence distribution of 3HB and 4HB units by analysis of the 125 MHz 13C n.r.m. spectra.
Yield of poly‐D(‐)‐3‐hydroxybutyrate from various carbon sources: A theoretical study
  • T. Yamane
  • Chemistry, Medicine
    Biotechnology and bioengineering
  • 1993
The theoretical yield of poly‐D(‐)‐3‐hydroxybutyrate (PHB) has been estimated from the biochemical pathway leading to PHB when a carbohydrate (glucose), a C1 compound, a C2 compound, or a C4 compound is used as a carbon source.
Hyperproduction of Poly-β-Hydroxybutyrate during Exponential Growth of Azotobacter vinelandii UWD
  • W. Page, O. Knosp
  • Biology, Medicine
    Applied and environmental microbiology
  • 1989
Strain UWD appeared to from poly-beta-hydroxybutyrate under novel conditions, which may be useful in designing new methods for the industrial production of biodegradable plastics.