Accommodation of foreign genes into the Sendai virus genome: sizes of inserted genes and viral replication

@article{Sakai1999AccommodationOF,
  title={Accommodation of foreign genes into the Sendai virus genome: sizes of inserted genes and viral replication},
  author={Yuko Sakai and Katsuhiro Kiyotani and Masayuki Fukumura and Makoto Asakawa and Atsushi Kato and Tatsuo Shioda and Tetsuya Yoshida and Akemi Tanaka and Mamoru Tsukuba-shi Hasegawa and Yoshiyuki Nagai},
  journal={FEBS Letters},
  year={1999},
  volume={456}
}
Sendai virus (SeV) is an enveloped virus with a negative sense genome RNA of about 15.3 kb. We previously established a system to recover an infectious virus entirely from SeV cDNA and illustrated the feasibility of using SeV as a novel expression vector. Here, we have attempted to insert a series of foreign genes into SeV of different lengths to learn how far SeV can accommodate extra genes and how the length of inserted genes affects viral replication in cells cultured in vitro and in the… 
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