Accidental selection and intentional restoration of sporulation-deficient Bacillus anthracis mutants.

@article{Sastalla2010AccidentalSA,
  title={Accidental selection and intentional restoration of sporulation-deficient Bacillus anthracis mutants.},
  author={Inka Sastalla and Mary J Rosovitz and Stephen H Leppla},
  journal={Applied and environmental microbiology},
  year={2010},
  volume={76 18},
  pages={
          6318-21
        }
}
We demonstrate the frequent accidental enrichment of spontaneous sporulation-deficient mutants of Bacillus anthracis on solid medium and identify contributing factors. Mutations in spo0A, encoding the master regulator of sporulation initiation, were found in 38 of 53 mutants. Transductions using bacteriophage CP51 propagated on sporogenic bacteria allowed for the restoration of sporulation phenotypes. 

Similar Papers

Figures, Tables, and Topics from this paper.

Explore Further: Topics Discussed in This Paper

References

Publications referenced by this paper.
SHOWING 1-10 OF 27 REFERENCES

Transducing bacteriophage for Bacillus cereus.

  • Journal of virology
  • 1968
VIEW 3 EXCERPTS
HIGHLY INFLUENTIAL

Genetic aspects of bacterial endospore formation.

  • Bacteriological reviews
  • 1976
VIEW 12 EXCERPTS
HIGHLY INFLUENTIAL

Comparisons of bacterial lag phases (Tlag) and doubling times (Td) of strains grown in LB a Strain Tlag (min) Td (min) Sterne 34F2

H. Maughan, J. Masel, C. W. Birky, Jr., W. L. Nicholson
  • SASTALLA ET AL. APPL. ENVIRON. MICROBIOL. on July 31,
  • 2007
VIEW 1 EXCERPT