Accessory cells with a morphology and marker pattern of dendritic cells can be obtained from elutriator-purified blood monocyte fractions. An enhancing effect of metrizamide in this differentiation.

@article{Kabel1989AccessoryCW,
  title={Accessory cells with a morphology and marker pattern of dendritic cells can be obtained from elutriator-purified blood monocyte fractions. An enhancing effect of metrizamide in this differentiation.},
  author={Peter J Kabel and Meeny de Haan-Meulman and Heronimus A M Voorbij and M Kleingeld and E F Knol and Hemmo A. Drexhage},
  journal={Immunobiology},
  year={1989},
  volume={179 4-5},
  pages={395-41}
}
Human monocytic cell fractions obtained by counterflow elutriation centrifugation (with regard to specific monocyte/macrophage characteristics: 82-88% were positive for nonspecific esterase; 86-94% for CD14) were cultured (overnight, 37 degrees C) under nonadhering conditions (polypropylene tubes). Thirty to 40% of the cells were found to differentiate into large, monocytoid cells with a dendritic morphology. These cells expressed a marker of active dendritic cells RFD1 in 76-89% and were also… CONTINUE READING