Accessibility of 18S rRNA in human 40S subunits and 80S ribosomes at physiological magnesium ion concentrations--implications for the study of ribosome dynamics.

@article{Shenvi2005AccessibilityO1,
  title={Accessibility of 18S rRNA in human 40S subunits and 80S ribosomes at physiological magnesium ion concentrations--implications for the study of ribosome dynamics.},
  author={Christina L Shenvi and Ken C. Dong and Eric M Friedman and Jeffrey A. Hanson and Jamie H D Cate},
  journal={RNA},
  year={2005},
  volume={11 12},
  pages={1898-908}
}
Protein biosynthesis requires numerous conformational rearrangements within the ribosome. The structural core of the ribosome is composed of RNA and is therefore dependent on counterions such as magnesium ions for function. Many steps of translation can be compromised or inhibited if the concentration of Mg(2+) is too low or too high. Conditions previously used to probe the conformation of the mammalian ribosome in vitro used high Mg(2+) concentrations that we find completely inhibit… CONTINUE READING
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