Accelerating the rate of disassembly of karyopherin.cargo complexes.

  title={Accelerating the rate of disassembly of karyopherin.cargo complexes.},
  author={Daniel Gilchrist and Brook Mykytka and Michael Rexach},
  journal={The Journal of biological chemistry},
  volume={277 20},
Transport of macromolecules across the nuclear pore complex (NPC) occurs in seconds and involves assembly of a karyopherin.cargo complex and docking to the NPC, translocation of the complex across the NPC via interaction with nucleoporins (Nups), and dissociation of the complex in the nucleoplasm. To identify rate-limiting steps in the Kap95p.Kap60p-mediated nuclear import pathway of Saccharomyces cerevisiae, we reconstituted key intermediate complexes and measured their rates of dissociation… CONTINUE READING