Accelerated production of antigen-specific T cells for preclinical and clinical applications using gas-permeable rapid expansion cultureware (G-Rex).

@article{Vera2010AcceleratedPO,
  title={Accelerated production of antigen-specific T cells for preclinical and clinical applications using gas-permeable rapid expansion cultureware (G-Rex).},
  author={Juan Francisco L{\'o}pez Vera and Lara J Brenner and Ulrike Gerdemann and Minhtran Charlotte Ngo and Uluhan Sili and Hao Liu and John Wilson and Gianpietro Dotti and Helen E Heslop and Ann M. Leen and Cliona M Rooney},
  journal={Journal of immunotherapy},
  year={2010},
  volume={33 3},
  pages={305-15}
}
The clinical manufacture of antigen-specific cytotoxic T lymphocytes (CTLs) for adoptive immunotherapy is limited by the complexity and time required to produce large numbers with the desired function and specificity. The culture conditions required are rigorous, and in some cases only achieved in 2-cm wells in which cell growth is limited by gas exchange, nutrients, and waste accumulation. Bioreactors developed to overcome these issues tend to be complex, expensive, and not always conducive to… CONTINUE READING