Accelerated production and identification of fertile, homozygous transgenic wheat lines by anther culture

@article{Massiah2004AcceleratedPA,
  title={Accelerated production and identification of fertile, homozygous transgenic wheat lines by anther culture},
  author={Andrea J. Massiah and Hong Rong and Sherralyn J Brown and Sophie A. Laurie},
  journal={Molecular Breeding},
  year={2004},
  volume={7},
  pages={163-173}
}
Anther culture has been developed in the winter wheat cultivar Florida to achieve accelerated production and identification of homozygous transgenic lines. With untransformed, seed-derived plants to develop the culture system, it was shown that cold pre-treatment of spikes excised from donor plants and addition of 2,4-dichlorophenoxyacetic acid together with either kinetin or 6-benzylaminopurine in the callus induction medium improves the anther culture response. The procedure developed allowed… CONTINUE READING