Accelerated bioorthogonal conjugation: a practical method for the ligation of diverse functional molecules to a polyvalent virus scaffold.

@article{Gupta2005AcceleratedBC,
  title={Accelerated bioorthogonal conjugation: a practical method for the ligation of diverse functional molecules to a polyvalent virus scaffold.},
  author={Sayam Sen Gupta and Jane Kuzelka and Pratik Singh and Warren G Lewis and M. Addison Manchester and M G Finn},
  journal={Bioconjugate chemistry},
  year={2005},
  volume={16 6},
  pages={1572-9}
}
Covalent bond formation to proteins is made difficult by their multiple unprotected functional groups and normally low concentrations. A water-soluble sulfonated bathophenanthroline ligand (2) was used to promote a highly efficient Cu(I)-mediated azide-alkyne cycloaddition (CuAAC) reaction for the chemoselective attachment of biologically relevant molecules to cowpea mosaic virus (CPMV). The ligated substrates included complex sugars, peptides, poly(ethylene oxide) polymers, and the iron… CONTINUE READING