Abundant expression and purification of biologically active mitochondrial citrate carrier in baculovirus-infected insect cells

  title={Abundant expression and purification of biologically active mitochondrial citrate carrier in baculovirus-infected insect cells},
  author={Marianna Madeo and Chiara Carrisi and Domenico Iacopetta and Loredana Capobianco and Anna Rita Cappello and Cecilia Bucci and Ferdinando Palmieri and Giancarlo Mazzeo and Anna Montalto and Vincenza Dolce},
  journal={Journal of Bioenergetics and Biomembranes},
Heterologous expression of recombinant proteins is an essential technology for protein characterization. A major obstacle to investigating the biochemical properties of membrane proteins is the difficulty in obtaining sufficient amounts of functional protein. Here we report the successful expression of the tricarboxylate (or citrate) carrier (CIC) of eel (Anguilla anguilla) from Spodoptera frugiperda (Sf9) cells using the baculovirus expression system. The recombinant CIC was purified by… 
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