Absorption spectral change of peripheral-light harvesting complexes 2 induced by magnesium protoporphyrin IX monomethyl ester association.

  title={Absorption spectral change of peripheral-light harvesting complexes 2 induced by magnesium protoporphyrin IX monomethyl ester association.},
  author={Huiying Yue and Chun-gui Zhao and Kai Li and Suping Yang},
  journal={Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy},
Several spectrally different types of peripheral light harvesting complexes (LH) have been reported in anoxygenic phototrophic bacteria in response to environmental changes. In this study, two spectral forms of LH2 (T-LH2 and U-LH2) were isolated from Rhodobacter azotoformans. The absorption of T-LH2 was extremely similar to the LH2 isolated from Rhodobacter sphaeroides. U-LH2 showed an extra peak at ∼423 nm in the carotenoid region. To explore the spectral origin of this absorption peak, the… Expand


Composition and localization of bacteriochlorophyll a intermediates in the purple photosynthetic bacterium Rhodopseudomonas sp. Rits
HPLC analyses of extracted pigments indicated that all four BChls a were associated with the purified photosynthetic pigment–protein, as complexes described above, which suggested that this bacterium could use these pigments as functional molecules within the LH2/4 and RC-LH1 core. Expand
Proteomic analysis of the developing intracytoplasmic membrane in Rhodobacter sphaeroides during adaptation to low light intensity.
A proteomic analysis of the intracytoplasmic membrane (ICM) assembly process during adaptation to lowered light intensity, in which the size of the photosynthetic units is greatly expanded by addition of the light-harvesting 2 (LH2) peripheral antenna complex. Expand
Differential carotenoid composition of the B875 and B800-850 photosynthetic antenna complexes in Rhodobacter sphaeroides 2.4.1: involvement of spheroidene and spheroidenone in adaptation to changes in light intensity and oxygen availability
It is demonstrated that in wild-type cells, spheroidene is predominantly associated with the B800-850 photosynthetic antenna complex andSpheroidenone is more abundant in the B875 complex. Expand
Preferential Incorporation of Coloured-carotenoids Occurs in the LH2 Complexes From Non-sulphur Purple Bacteria Under Carotenoid-limiting Conditions
The results show that although the presence of Car molecules is important for the stability of the LH2 complexes that the overall native structure can be maintained by the absence of many different Cars. Expand
Differential assembly of polypeptides of the light-harvesting 2 complex encoded by distinct operons during acclimation of Rhodobacter sphaeroides to low light intensity
A proteomic analysis of pigment-protein complexes isolated from the purple bacterium Rhodobacter sphaeroides undergoing acclimation to reduced incident light intensity revealed that the LH2 band contained distinct levels of the LH1-α and -β polypeptides encoded by the two puc operons, consistent with the origin of this band from a pool of LH2 laid down early in development that is distinct from subsequently assembled LH2-only domains. Expand
Regulation of tetrapyrrole synthesis by light in chemostat cultures of Rhodobacter sphaeroides
  • J. Oelze
  • Biology, Medicine
  • Journal of bacteriology
  • 1988
Control of bacteriochlorophyll, magnesium protoporphyrin monomethyl ester, cytochromes, and coproporphyrine by light was studied with chemostat cultures of Rhodobacter sphaeroides, suggesting that light controls a second step of MgPME conversion whenever too much precursor is available, owing to the low sensitivity of the initial step of control. Expand
Spectral properties of single light-harvesting complexes in bacterial photosynthesis
Photosynthetic light-harvesting complexes play important roles in the early events in photosynthesis in nature, and have been studied by various spectroscopic methodologies. Single moleculeExpand
The nature of a pgiment-protein complex excreted from mutants of Rhodopseudomonas sphaeroides.
Pigment-protein complexes excreted from three bacteriochlorophyll-less mutants (mutants 8, 8-29, and 8-47) of Rhodopseudomonas sphaeroides have been isolated and purified. In the absence ofExpand
Aerobic and Anaerobic Mg-Protoporphyrin Monomethyl Ester Cyclases in Purple Bacteria
Two different mechanisms for Mg-protoporphyrin monomethyl ester (MgPMe) cyclization are shown to coexist in Rubrivivax gelatinosus and are proposed to be conserved in all facultative aerobicExpand
BchJ and BchM interact in a 1 : 1 ratio with the magnesium chelatase BchH subunit of Rhodobacter capsulatus
Results suggest that BchJ may play a role as a general magnesium porphyrin carrier, similar to one of the roles of GUN4 in oxygenic organisms. Expand