Absorption of Recombinant Human Granulocyte Colony-Stimulating Factor (rhG-CSF) from Rat Nasal Mucosa

@article{Machida2004AbsorptionOR,
  title={Absorption of Recombinant Human Granulocyte Colony-Stimulating Factor (rhG-CSF) from Rat Nasal Mucosa},
  author={M. Machida and K. Sano and M. Arakawa and M. Hayashi and S. Awazu},
  journal={Pharmaceutical Research},
  year={2004},
  volume={10},
  pages={1372-1377}
}
Nasal absorption of recombinant human granulocyte colony-stimulating factor (rhG-CSF) was examined in the rat. The relative bioavailability of rhG-CSF for subcutaneous administration was ∼2%, as evaluated from the immunologically active rhG-CSF concentration in rat plasma and the area under the curve (AUC) of the plasma rhG-CSF concentration versus time for 8 hr. Pharmacological availability relative to subcutaneous administration was determined from the increase in total blood leukocyte… Expand
Pulmonary Delivery of Powders and Solutions Containing Recombinant Human Granulocyte Colony-Stimulating Factor (rhG-CSF) to the Rabbit
TLDR
Two powder formulations (MMAD <4 µm) containing rhG-CSF were insufflated (IF) via an endotracheal tube at doses of 5, 75 or 500 µg/kg to New Zealand white rabbits, indicating that rhG -CSF retains activity in the solid state. Expand
Preparation and characterisation of PLGA microspheres for sustained release of recombinant human granulocyte colony-stimulating factor
The purpose of this research was to develop a sustained micosphere for recombinant human granulocyte colony-stimulating factor (RHG-CSF). RHG-CSF was first incorporated into glassy sodium hyaluronateExpand
Design of long-acting formulation of protein drugs with a double-layer structure and its application to rhG-CSF.
TLDR
The results of this study indicated that DL-MP was useful as a long-acting formulation of rhG-CSF characterized by excellent long acting properties without an initial burst. Expand
Sustained-release G-CSF microspheres using a novel solid-in-oil-in-oil-in-water emulsion method
TLDR
Results suggested that the microspheres prepared by the S/O/ O/W method had increased neutrophil activity compared to those prepared by W/O-W. Expand
Surgical wound healing using hemostatic gauze scaffold loaded with nanoparticles containing sustained-release granulocyte colony-stimulating factor
TLDR
In this study, a formulation of hemostatic gauze impregnated with sustained-release granulocyte colony-stimulating factor (G-CSF) with increasing of the neutrophilic granulocytes count in the blood following chemotherapy and decreasing blood loss after surgical tumor resection is developed. Expand
Factores que intervienen en el diseño de sistemas de liberación nasal de péptidos y proteínas
Factors involved in the design of nasal delivery systems for peptides and proteins. Because the nasal mucous is one of the most permeable areas, the intranasal delivery route is the most promisingExpand
Proteins as Drugs: Analysis, Formulation and Delivery
The use of proteins as drugs is by no means new. Insulin, gamma-globulin and protein-containing vaccines have been routinely employed for decades. However, the advent of recombinant DNA technologyExpand
Advanced formulations for intranasal delivery of biologics
TLDR
The nasal epithelium is a promising route for biologics administration, which is reflected in a number of well‐established products on the market treating chronic diseases as well as a large number of clinical trials currently in progress. Expand
Treatment of systemic diseases by inhalation of biomolecule aerosols.
  • R. Siekmeier, G. Scheuch
  • Medicine
  • Journal of physiology and pharmacology : an official journal of the Polish Physiological Society
  • 2009
TLDR
This review describes some examples other than insulin in the field of inhalant treatment of systemic diseases that have been introduced in clinical treatment for a short time and a lot of other molecules have been tested in aerosol inhalation studies. Expand
Absorption Enhancers for Nasal Drug Delivery
TLDR
The basic concepts for the transmucosal delivery of drugs, and in particular the use of the nasal route for delivery of challenging drugs such as polar low-molecular-weight drugs and peptides and proteins, are described. Expand
...
1
2
...

References

SHOWING 1-10 OF 11 REFERENCES
Purification and characterization of human granulocyte colony‐stimulating factor (G‐CSF).
A colony‐stimulating factor (CSF) has been purified to homogeneity from the serum‐free medium conditioned by one of the human CSF‐producing tumor cell lines, CHU‐2. The molecule was a hydrophobicExpand
Quantitative enzyme immunoassay for human granulocyte colony stimulating factor (G-CSF).
TLDR
Human granulocyte colony stimulating factor (G-CSF) was detected at the pM level by a simple sequential sandwich enzyme immunoassay and gave satisfactory reproducibility as judged. Expand
Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor
TLDR
Southern hybridization analysis of DNA from normal leukocytes and CHU-2 cells suggests that the human genome contains only one gene for G-CSF and that some rearrangement has occurred within one of the alleles of the G- CSF gene in CHU -2 cells. Expand
Structures of the sugar chains of recombinant human granulocyte-colony-stimulating factor produced by Chinese hamster ovary cells.
TLDR
Recombinant human granulocyte-colony-stimulating factor was purified from Chinese hamster ovary cells transfected with human G-CSF cDNA and two oligosaccharide-alditols obtained and their structures were identified by component analysis and 500-MHz 1H-NMR spectroscopy. Expand
Absorption of recombinant methionyl-human growth hormone (Met-hGH) from rat nasal mucosa
Abstract The absorption of recombinant methionyl human growth hormone (Met-hGH) from the nasal mucosa into the systemic circulation was studied in anesthetized rats. Met-hGH was administeredExpand
Protective activity of recombinant cytokines against Sendai virus and herpes simplex virus (HSV) infections in mice.
The efficacy of recombinant cytokines such as murine interferon-gamma (IFN-gamma), human granulocyte colony-stimulating factor (G-CSF), mouse granulocytic-macrophage colony-stimulating factorExpand
Absorption of drugs from the nasal mucosa of rat
Abstract Absorption of drugs through the nasal mucosa was studied to find out the suitability of the nasal mucosa as a new site of administration. The in situ recirculation test with the nasal cavityExpand
Analysis of structural requirements for the absorption of drugs and macromolecules from the nasal cavity.
TLDR
It is argued that the most likely route for transport is through junctions between cells and that surface-active adjuvants which markedly enhance insulin uptake may act by rendering hydrophobic areas of contact of the junctional proteins temporarily hydrophilic. Expand
Effect of surfactants on the nasal absorption of insulin in rats
TLDR
The absorption of insulin through the nasal mucosa was enhanced when a surfactant, among various non-ionic, anionic and amphoteric surfactants, including the bile acid salts, saponin and peptidelipid (surfactin), was added to the insulin solution. Expand
The Molecular Weight Dependence of Nasal Absorption: The Effect of Absorption Enhancers
TLDR
Results indicate that the paracellular routes may play an important role in large molecule absorption through the nasal mucosa when changes in cell-to-cell adhesion in the mucosa are observed. Expand
...
1
2
...