Absorption of Recombinant Human Granulocyte Colony-Stimulating Factor (rhG-CSF) from Rat Nasal Mucosa

  title={Absorption of Recombinant Human Granulocyte Colony-Stimulating Factor (rhG-CSF) from Rat Nasal Mucosa},
  author={M. Machida and K. Sano and M. Arakawa and M. Hayashi and S. Awazu},
  journal={Pharmaceutical Research},
Nasal absorption of recombinant human granulocyte colony-stimulating factor (rhG-CSF) was examined in the rat. The relative bioavailability of rhG-CSF for subcutaneous administration was ∼2%, as evaluated from the immunologically active rhG-CSF concentration in rat plasma and the area under the curve (AUC) of the plasma rhG-CSF concentration versus time for 8 hr. Pharmacological availability relative to subcutaneous administration was determined from the increase in total blood leukocyte… Expand
Pulmonary Delivery of Powders and Solutions Containing Recombinant Human Granulocyte Colony-Stimulating Factor (rhG-CSF) to the Rabbit
Two powder formulations (MMAD <4 µm) containing rhG-CSF were insufflated (IF) via an endotracheal tube at doses of 5, 75 or 500 µg/kg to New Zealand white rabbits, indicating that rhG -CSF retains activity in the solid state. Expand
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Treatment of systemic diseases by inhalation of biomolecule aerosols.
  • R. Siekmeier, G. Scheuch
  • Medicine
  • Journal of physiology and pharmacology : an official journal of the Polish Physiological Society
  • 2009
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The basic concepts for the transmucosal delivery of drugs, and in particular the use of the nasal route for delivery of challenging drugs such as polar low-molecular-weight drugs and peptides and proteins, are described. Expand


Purification and characterization of human granulocyte colony‐stimulating factor (G‐CSF).
A colony‐stimulating factor (CSF) has been purified to homogeneity from the serum‐free medium conditioned by one of the human CSF‐producing tumor cell lines, CHU‐2. The molecule was a hydrophobicExpand
Quantitative enzyme immunoassay for human granulocyte colony stimulating factor (G-CSF).
Human granulocyte colony stimulating factor (G-CSF) was detected at the pM level by a simple sequential sandwich enzyme immunoassay and gave satisfactory reproducibility as judged. Expand
Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor
Southern hybridization analysis of DNA from normal leukocytes and CHU-2 cells suggests that the human genome contains only one gene for G-CSF and that some rearrangement has occurred within one of the alleles of the G- CSF gene in CHU -2 cells. Expand
Structures of the sugar chains of recombinant human granulocyte-colony-stimulating factor produced by Chinese hamster ovary cells.
Recombinant human granulocyte-colony-stimulating factor was purified from Chinese hamster ovary cells transfected with human G-CSF cDNA and two oligosaccharide-alditols obtained and their structures were identified by component analysis and 500-MHz 1H-NMR spectroscopy. Expand
Absorption of recombinant methionyl-human growth hormone (Met-hGH) from rat nasal mucosa
Abstract The absorption of recombinant methionyl human growth hormone (Met-hGH) from the nasal mucosa into the systemic circulation was studied in anesthetized rats. Met-hGH was administeredExpand
Protective activity of recombinant cytokines against Sendai virus and herpes simplex virus (HSV) infections in mice.
The efficacy of recombinant cytokines such as murine interferon-gamma (IFN-gamma), human granulocyte colony-stimulating factor (G-CSF), mouse granulocytic-macrophage colony-stimulating factorExpand
Absorption of drugs from the nasal mucosa of rat
Abstract Absorption of drugs through the nasal mucosa was studied to find out the suitability of the nasal mucosa as a new site of administration. The in situ recirculation test with the nasal cavityExpand
Analysis of structural requirements for the absorption of drugs and macromolecules from the nasal cavity.
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Effect of surfactants on the nasal absorption of insulin in rats
The absorption of insulin through the nasal mucosa was enhanced when a surfactant, among various non-ionic, anionic and amphoteric surfactants, including the bile acid salts, saponin and peptidelipid (surfactin), was added to the insulin solution. Expand
The Molecular Weight Dependence of Nasal Absorption: The Effect of Absorption Enhancers
Results indicate that the paracellular routes may play an important role in large molecule absorption through the nasal mucosa when changes in cell-to-cell adhesion in the mucosa are observed. Expand