Absorption and scattering correction in fluorescence confocal microscopy

  title={Absorption and scattering correction in fluorescence confocal microscopy},
  author={T. Visser and F. Groen and G. Brakenhoff},
  journal={Journal of Microscopy},
In three‐dimensional (3‐D) fluorescence images produced by a confocal scanning laser microscope (CSLM), the contribution of the deeper layers is attenuated due to absorption and scattering of both the excitation and the fluorescence light. Because of these effects a quantitative analysis of the images is not always possible without restoration. Both scattering and absorption are governed by an exponential decay law. Using only one (space‐dependent) extinction coefficient, the total attenuation… Expand
An FFT‐based method for attenuation correction in fluorescence confocal microscopy
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Robust incremental compensation of the light attenuation with depth in 3 D fluorescence microscopy
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Quantifying fluorescence signals in confocal image stacks deep in turbid media
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Robust incremental compensation of the light attenuation with depth in 3D fluorescence microscopy
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Using an electromagnetic approach, we calculate the properties of a confocal fluorescence microscope. It is expected that the results will be more reliable than those obtained by conventional scalarExpand
Image heterogeneity correction in large-area, three-dimensional multiphoton microscopy.
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Attenuation correction in confocal laser microscopes: a novel two‐view approach
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Confocal scanning light microscopy with high aperture immersion lenses
The imaging characteristics of a confocal scanning light microscope (CSLM) with high aperture, immersion type, lenses (N.A. = 1·3) are investigated. In the confocal arrangement the images of theExpand
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This project was partly sponsored by the SPIN 3-D Image Analysis Project (Stimulation Program Computer Science Netherlands)