Absorption and scattering correction in fluorescence confocal microscopy

@article{Visser1991AbsorptionAS,
  title={Absorption and scattering correction in fluorescence confocal microscopy},
  author={Taco D. Visser and Frans C. A. Groen and G. J. Brakenhoff},
  journal={Journal of Microscopy},
  year={1991},
  volume={163}
}
In three‐dimensional (3‐D) fluorescence images produced by a confocal scanning laser microscope (CSLM), the contribution of the deeper layers is attenuated due to absorption and scattering of both the excitation and the fluorescence light. Because of these effects a quantitative analysis of the images is not always possible without restoration. Both scattering and absorption are governed by an exponential decay law. Using only one (space‐dependent) extinction coefficient, the total attenuation… 

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This project was partly sponsored by the SPIN 3-D Image Analysis Project (Stimulation Program Computer Science Netherlands)