Absolute quantitation of feline leukemia virus proviral DNA and viral RNA loads by TaqMan real-time PCR and RT-PCR.

Abstract

Sensitive TaqMan real-time polymerase chain reaction (PCR)-based methods have been developed recently for the detection and quantitation of feline leukemia virus (FeLV) proviral DNA in infected cats. In this chapter, we outline the design and implementation of a TaqMan real-time PCR assay to quantify total FeLV proviral and viral RNA loads in infected cats… (More)
DOI: 10.1007/978-1-60327-040-3_6

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