Absolute quantification of the glycolytic pathway in yeast: deployment of a complete QconCAT approach.

@article{Carroll2011AbsoluteQO,
  title={Absolute quantification of the glycolytic pathway in yeast: deployment of a complete QconCAT approach.},
  author={Kathleen M. Carroll and Deborah M Simpson and Claire E Eyers and Christopher T. G. Knight and Philip J. Brownridge and Warwick B. Dunn and Catherine Winder and Karin Lanthaler and Pinar Pir and Naglis Malys and Douglas B. Kell and Stephen G. Oliver and Simon J. Gaskell and Robert J Beynon},
  journal={Molecular & cellular proteomics : MCP},
  year={2011},
  volume={10 12},
  pages={M111.007633}
}
The availability of label-free data derived from yeast cells (based on the summed intensity of the three strongest, isoform-specific peptides) permitted a preliminary assessment of protein abundances for glycolytic proteins. Following this analysis, we demonstrate successful application of the QconCAT technology, which uses recombinant DNA techniques to generate artificial concatamers of large numbers of internal standard peptides, to the quantification of enzymes of the glycolysis pathway in… CONTINUE READING