Absolute quantification of microRNAs by using a universal reference.

  title={Absolute quantification of microRNAs by using a universal reference.},
  author={Ute Bissels and Stefan Wild and Stefan Tomiuk and Angela Holste and Markus Hafner and Thomas Tuschl and Andreas Bosio},
  volume={15 12},
MicroRNAs (miRNAs) are a species of small RNAs approximately 21-23-nucleotides long that have been shown to play an important role in many different cellular, developmental, and physiological processes. Accordingly, numerous PCR-, sequencing-, or hybridization-based methods have been established to identify and quantify miRNAs. Their short length results in a high dynamic range of melting temperatures and therefore impedes a proper selection of detection probes or optimized PCR primers. While… CONTINUE READING

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