Absolute proteomic quantification of the activity state of proteases and proteolytic cleavages using proteolytic signature peptides and isobaric tags.

@article{Fahlman2014AbsolutePQ,
  title={Absolute proteomic quantification of the activity state of proteases and proteolytic cleavages using proteolytic signature peptides and isobaric tags.},
  author={Richard Peter Fahlman and Wei Chen and Christopher M. Overall},
  journal={Journal of proteomics},
  year={2014},
  volume={100},
  pages={79-91}
}
UNLABELLED Proteolytic processing alters the structure and function of a wide range of proteins in the proteome. We describe a method for the absolute quantification of proteolysis that is compatible with existing quantitative proteomic applications and could be applied on a protein-family wide scale. A tryptic peptide spanning a cleavage site differentiates this intact form of the protein from the corresponding semi-tryptic peptides of a protease cleaved protein. We term such proteomic… CONTINUE READING