Absence of stable intermediates on the folding pathway of barnase.

  title={Absence of stable intermediates on the folding pathway of barnase.},
  author={Jiro Takei and R A Chu and Yawen Bai},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={97 20},
Barnase is one of the few protein models that has been studied extensively for protein folding. Previous studies led to the conclusion that barnase folds through a very stable submillisecond intermediate ( approximately 3 kcal/mol). The structure of this intermediate was characterized intensively by using a protein engineering approach. This intermediate has now been reexamined with three direct and independent methods. (i) Hydrogen exchange experiments show very small protection factors… CONTINUE READING