Absence of cumulus cells during in vitro maturation affects lipid metabolism in bovine oocytes.

  title={Absence of cumulus cells during in vitro maturation affects lipid metabolism in bovine oocytes.},
  author={Sylvain Auclair and Rustem E. Uzbekov and S{\'e}bastien Elis and Laura Sanchez and Igor I. Kireev and Lionel Lardic and Rozenn Dalbies-Tran and Svetlana Uzbekova},
  journal={American journal of physiology. Endocrinology and metabolism},
  volume={304 6},
Cumulus cells (CC) surround the oocyte and are coupled metabolically through regulation of nutrient intake. CC removal before in vitro maturation (IVM) decreases bovine oocyte developmental competence without affecting nuclear meiotic maturation. The objective was to investigate the influence of CC on oocyte cytoplasmic maturation in relation to energy metabolism. IVM with either cumulus-enclosed (CEO) or -denuded (DO) oocytes was performed in serum-free metabolically optimized medium… 

Response of Bovine Cumulus–Oocytes Complexes to Energy Pathway Inhibition during In Vitro Maturation

The results indicate that only glucose metabolism is substantial to the oocyte during IVM since only glucose inhibition decreased embryo culture efficiency and it is suggested that the energy pathways analyzed in this experiment are not interchangeable alternatives in bovine COCs.

Fatty acid synthesis and oxidation in cumulus cells support oocyte maturation in bovine.

FAs metabolism in CCs is important to maintain metabolic homeostasis and may influence meiosis progression and survival of enclosed oocytes.

Differences in cumulus cell gene expression indicate the benefit of a pre-maturation step to improve in-vitro bovine embryo production.

Based on the analysis of CC transcriptomic profiles and the relationship between morphological features of the COC and the oocyte chromatin configuration, a customized, stage-dependent oocyte pre-maturation (pre-IVM) system was used to improve oocyte developmental potential before IVM.

Tribbles expression in cumulus cells is related to oocyte maturation and fatty acid metabolism

Investigation of Tribbles expression patterns in bovine CC during in vitro maturation (IVM) and their roles in the cumulus-oocyte complex found Tribbles family members may be involved in cell proliferation and in FAO signaling in CC and participate in oocyte meiotic resumption regulation.

MALDI mass spectrometry reveals that cumulus cells modulate the lipid profile of in vitro-matured bovine oocytes

Results indicate a modulation by CC of the oocyte TAG and PL profiles associated with a specific cell response to the serum supplement used for in vitro maturation.

Effects of Hyaluronidase during In Vitro Maturation on Maturation and Developmental Competence in Porcine Oocytes

It is suggested that suppression of cumulus expansion of COCs could induce oxidative stress and decrease nuclear maturation via reduction of GSH synthesis and it caused to decrease developmental competence of mammalian oocytes.

Effects of different stimulators of cGMP synthesis on lipid content in bovine oocytes matured in vitro

Abstract Bovine oocytes and blastocysts produced in vitro are frequently of lower quality and less cryotolerant than those produced in vivo, and greater accumulation of lipids in the cytoplasm has

Melatonin Regulates Lipid Metabolism in Porcine Cumulus–Oocyte Complexes via the Melatonin Receptor 2

Understanding the dynamics of the interactions between lipid homeostasis and redox signaling driven by MT2 is necessary in order to predict drug targets and the effects of therapeutics used to improve female reproductive health.

Maturation system affects lipid accumulation in bovine oocytes.

It is indicated, for the first time, that oocytes matured by IFIOT are similar to those matured invivo with regard to lipid accumulation, which indicates better quality than those maturedInvitro.

Gene expression in human cumulus cells: one approach to oocyte competence.

Nuclear maturation was thus associated with increased expression of STAR, COX2, AREG, SCD1 and SCD5 by cumulus cells and further developmental potential from embryo to blastocyst stage was associated with lower expression in a narrow range for these genes.

Beta-Oxidation Is Essential for Mouse Oocyte Developmental Competence and Early Embryo Development1

The importance of lipid metabolism for oocyte developmental competence and early embryo development was demonstrated by assessing the rate of embryo development following inhibition or upregulation of beta-oxidation with etomoxir (an inhibitor of CPT1B) or l-carnitine, respectively.

The pivotal role of glucose metabolism in determining oocyte developmental competence.

The relevant pathways of glucose metabolism by COCs during in vivo maturation and IVM are discussed, including the relative contribution of the somatic and gamete compartments of the COC to glucose metabolism.

Oocyte developmental failure in response to elevated nonesterified fatty acid concentrations: mechanistic insights.

Data show that the degree of mitochondrial fatty acid β-oxidation has a decisive impact on the development of NEFA-exposed oocytes, and gene expression data suggest that the resulting embryos adapt through altered metabolic strategies, which might explain the aberrant energy metabolism previously observed in these embryos originating from NEFA -exposed maturing oocytes.

Oocyte-secreted factors: regulators of cumulus cell function and oocyte quality.

A new perspective on oocyte-CC interactions is improving knowledge of the processes regulating oocyte quality, which is likely to have a number of applications, including improving the efficiency of clinical IVM and thereby providing new options for the treatment of infertility.

Enhanced glycolysis after maturation of bovine oocytes in vitro is associated with increased developmental competence

Metabolism of glucose through the Embden‐Meyerhof pathway is important during oocyte maturation in vitro, and higher glycolytic rates in in vitro matured oocytes may reflect increased developmental competence.

Effects of Sodium Pyruvate in Nonserum Maturation Medium on Maturation, Fertilization, and Subsequent Development of Bovine Oocytes With or Without Cumulus Cells

It is indicated that sodium pyruvate promotes nuclear maturation of bovine CDOs and that a continuing presence of cumulus cells during maturation is important for subsequent development of zygotes to the blastocyst stage.