Absence of Protein Kinase C in Nuclei of EL 4 Mouse Thymoma Cells 1

Since evidence indicates that phorbol ester-induced production of interleukin 2 requires transcription, we investigated the possibility that the phorbol ester receptor acts directly in the nuclei of EL4 thymoma cells. Using a procedure that minimized plasma membrane contamination (as measured by S'-nucleotidase activity) and maintained the integrity of the… CONTINUE READING