Abscisic acid binding to subcellular fractions from leaves of Vicia faba

@article{Hocking1978AbscisicAB,
  title={Abscisic acid binding to subcellular fractions from leaves of Vicia faba},
  author={T. J. Hocking and Jennifer Clapham and K. J. Cattell},
  journal={Planta},
  year={1978},
  volume={138},
  pages={303-304}
}
A centrifugation binding assay has been used to demonstrate the binding of [3H] (±) abscisic acid to membrane-rich fractions prepared from leaves of Vicia faba L. Kinetic analysis of this binding shows evidence of saturation of binding sites with increasing concentration of ligand. Scatchard analysis of these data yields a biphasic plot possibly indicating the presence of two types of binding sites. The dissocation constant for the high affinity site has been calculated to be 3.5×10-8 mol 1-1. 

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Mechanics and Metabolism of Guard Cells

H. Meidner, C. Willmer
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Inhibition by abseisic acid of the light and dark uptake of potassium by slices of Vicia faba leaves

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