Abscisic acid binding to subcellular fractions from leaves of Vicia faba

  title={Abscisic acid binding to subcellular fractions from leaves of Vicia faba},
  author={T. J. Hocking and Jennifer Clapham and K. J. Cattell},
A centrifugation binding assay has been used to demonstrate the binding of [3H] (±) abscisic acid to membrane-rich fractions prepared from leaves of Vicia faba L. Kinetic analysis of this binding shows evidence of saturation of binding sites with increasing concentration of ligand. Scatchard analysis of these data yields a biphasic plot possibly indicating the presence of two types of binding sites. The dissocation constant for the high affinity site has been calculated to be 3.5×10-8 mol 1-1. 

From This Paper

Figures, tables, and topics from this paper.


Publications referenced by this paper.
Showing 1-7 of 7 references

Mechanics and Metabolism of Guard Cells

H. Meidner, C. Willmer
Curt . Adv . Plant Sci . • 1975

The Chemistry and Physiology of Abscisic Acid

B. V. Milborrow
Ann Rev . Plant Physiol . • 1974

Inhibition by abseisic acid of the light and dark uptake of potassium by slices of Vicia faba leaves

R. F. Horton, K. R. Bruce
Can . J . Bot . • 1972

Similar Papers

Loading similar papers…