Abrogation of the requirements for added growth factors in 3T3 cells constitutively expressing the p53 and IGF-1 genes.

Two constructs (co-transfected with selectable markers) were used to establish cell lines from BALB/c3T3 cells: a p53 mini-gene under the control of an LTR promoter and a synthetic IGF-1 coding sequence driven by the SV40 early promoter. BALB/c3T3 cells do not grow in plasma or in 1% serum or in soft agar and in defined media require both platelet-derived… (More)