Abrogated cryptic activation of lentiviral transfer vectors

  title={Abrogated cryptic activation of lentiviral transfer vectors},
  author={Ralf M. Luche and Joerg Enssle and Hans-Peter Kiem},
  booktitle={Scientific reports},
Despite significant improvements in lentivirus (LV) vector-based gene therapy there are still several safety risks using LV vectors including the potential formation of replication-competent LV particles. To address this shortcoming, we constructed a novel and safer gene transfer system using modified SIN-based LV gene transfer vectors. Central to our approach is a conditional deletion of the Ψ packaging signal after integration in the target genome. Here we demonstrate that after transduction… CONTINUE READING