Abortive infection of human diploid cells by murine cytomegalovirus.

  title={Abortive infection of human diploid cells by murine cytomegalovirus.},
  author={Kwang Sik Kim and Richard I. Carp},
  journal={Infection and immunity},
  volume={6 5},
Inoculation of human diploid cells (WI-38) with murine cytomegalovirus (MCMV) did not result in the synthesis of any infectious virions. However, morphological changes typical of the cytopathic effects (CPE) of MCMV were detectable within 12 hr of infection. The CPE included rounding, swelling, and detachment of cells. The nuclei of infected cells were enlarged, and intranuclear inclusions were visible by May Grunwald-Giemsa staining and by the indirect fluorescent-antibody test. All cells… CONTINUE READING