Abnormal spindle protein, Asp, and the integrity of mitotic centrosomal microtubule organizing centers.

@article{Avides1999AbnormalSP,
  title={Abnormal spindle protein, Asp, and the integrity of mitotic centrosomal microtubule organizing centers.},
  author={Maria do Carmo Avides and David M Glover},
  journal={Science},
  year={1999},
  volume={283 5408},
  pages={1733-5}
}
The product of the abnormal spindle (asp) gene was found to be an asymmetrically localized component of the centrosome during mitosis, required to focus the poles of the mitotic spindle in vivo. Removing Asp protein function from Drosophila melanogaster embryo extracts, either by mutation or immunodepletion, resulted in loss of their ability to restore microtubule-organizing center activity to salt-stripped centrosome preparations. This was corrected by addition of purified Asp protein. Thus… CONTINUE READING