Abnormal expression of TIMP-2, SOD, vimentin and PAI proteins in cloned bovine placentae.

@article{Kim2009AbnormalEO,
  title={Abnormal expression of TIMP-2, SOD, vimentin and PAI proteins in cloned bovine placentae.},
  author={H-R Kim and K. Naruse and H.-R. Lee and R. Han and C-S Park and D. Jin},
  journal={Reproduction in domestic animals = Zuchthygiene},
  year={2009},
  volume={44 4},
  pages={
          714-7
        }
}
  • H-R Kim, K. Naruse, +3 authors D. Jin
  • Published 2009
  • Biology, Medicine
  • Reproduction in domestic animals = Zuchthygiene
Cloned mammals suffer from high rates of placental abnormality and foetal loss during pregnancy. We previously used 2-D gel electrophoresis and mass spectrometry for global proteomic analysis of cloned and normal bovine placentae to identify differential protein expression patterns. Here, we used Western blot analysis to confirm the expression levels of several pregnancy-related proteins putatively identified as being differentially expressed in somatic cell nuclear transfer (SCNT) vs normal… Expand
Aberrant Expression of TIMP-2 and PBEF Genes in the Placentae of Cloned Mice Due to Epigenetic Reprogramming Error
TLDR
The results suggest that cloned placentae appear to suffer from failure of histone modification-based reprogramming in these (and potentially other) developmentally important genes, leading to aberrant expression of their protein products. Expand
Aberrant protein expression in the placenta of cloned mouse derived from embryonic stem cell.
Placentomegaly is a common phenotype in cloned mice. To assess differences in protein expression between placentae of cloned and uncloned mice, we used a proteomic approach involving 2-dimensionalExpand
Epigenetic characterization of the PBEF and TIMP-2 genes in the developing placentae of normal mice.
TLDR
It is suggested that epigenetic changes may regulate gene expression level in the developing placentae of normal mice and that inappropriate epigenetic reprogramming might be one cause of the abnormal placenta seen in cloned animals. Expand
Characterization of Bioactive Recombinant Human Lysozyme Expressed in Milk of Cloned Transgenic Cattle
TLDR
Transgenic cattle whose milk offers the similar nutritional benefits as human milk are described and techniques that could be further refined for production of active human lysozyme on a large scale are reported. Expand

References

SHOWING 1-10 OF 27 REFERENCES
Protein profiles of bovine placenta derived from somatic cell nuclear transfer
TLDR
A global proteomics approach using 2‐DE and MS revealed composite profiles of key proteins involved in abnormal placenta derived from SCNT, and suggested expression abnormality of these genes in SCNT placentA, resulting in fetal losses following SCNT. Expand
Matrix metalloproteinases (MMP-2 and MMP-9) and tissue inhibitor-2 of matrix metalloproteinases (TIMP-2) in the placenta and interplacental uterine wall in normal cows and in cattle with retention of fetal membranes.
Matrixmetalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) play a key role in tissue re-modelling in the placenta. In the present study, distribution of MMP-2, MMP-9Expand
Gene expression and methylation patterns in cloned embryos.
A considerable proportion of the offspring, in particular in ruminants and mouse, born from nuclear transfer (NT)-derived and in vitro-produced (IVP) embryos are affected by multiple abnormalities,Expand
Immunohistochemical assessment of superoxide dismutase expression in the endometrium in endometriosis and adenomyosis.
TLDR
The exaggerated expression of both SODs in the endometrium throughout the menstrual cycle suggests that superoxide plays a key role in infertility in endometriosis and adenomyosis. Expand
Changes in extracellular matrix components and cytokeratins in the endometrium during goat implantation.
TLDR
A local control of protease activity within the endometrium is suggested and major changes in the epithelial phenotype of the uterine cells during trophoblast adhesion in ruminants are demonstrated. Expand
Rescue of the Corpus Luteum and an Increase in Luteal Superoxide Dismutase Expression Induced by Placental Luteotropins in the Rat: Action of Testosterone Without Conversion to Estrogen1
TLDR
Placentae obtained from rats on Day 12 of pregnancy were incubated for 24 h, and the supernatant was used as placental luteotropins to increase SOD expression in the corpus luteum and stimulated progesterone production in pseudopregnant rats. Expand
Genome-Wide Epigenetic Alterations in Cloned Bovine Fetuses1
TLDR
This is the first report to indicate that global methylation losses may contribute to the developmental failure of cloned bovine fetuses and imply that survivability ofCloned cattle may be closely related to the global DNA methylation status. Expand
Matrix metalloproteinases and their inhibitors in connective tissue remodeling
  • J. Woessner
  • Biology, Medicine
  • FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 1991
TLDR
Latency is overcome by physical, chemical, and enzymatic treatments that separate the cysteine residue from the zinc Expression of the metalloproteinases is switched on by a variety of agents acting through regulatory elements of the gene, particularly the AP‐1 binding site. Expand
Evidence for Placental Abnormality as the Major Cause of Mortality in First-Trimester Somatic Cell Cloned Bovine Fetuses1
TLDR
The findings suggest that future research should focus on factors that promotes placental and vascular growth and on fetomaternal interactions that promote placental attachment and villous formation. Expand
Antioxidant systems in normal pregnancy and in pregnancy-induced hypertension.
TLDR
This article investigates whether changes in antioxidant systems contribute to pregnancy-induced hypertension by testing two extracellular (plasma thiols and ceruloplasmin) and two intracellular (red blood cell lysate thiolS and red blood cell superoxide dismutase) antioxidant markers. Expand
...
1
2
3
...