Aberrant protein expression in the placenta of cloned mouse derived from embryonic stem cell.

  title={Aberrant protein expression in the placenta of cloned mouse derived from embryonic stem cell.},
  author={Hong-Rye Kim and R. Han and T. Wakayama and Chang-Sik Park and D. Jin},
  volume={31 10},
Placentomegaly is a common phenotype in cloned mice. To assess differences in protein expression between placentae of cloned and uncloned mice, we used a proteomic approach involving 2-dimensional electrophoresis (DE) and MALDI-TOF MS. Proteins within isoelectric point range of 4-11 separately were analyzed in 2-DE with 3 replications of each sample. A total of approximately 3500 spots were detected in placental 2-DE stained with Coomassie blue. In the comparison of normal and cloned samples, a… Expand
Aberrant Expression of TIMP-2 and PBEF Genes in the Placentae of Cloned Mice Due to Epigenetic Reprogramming Error
The results suggest that cloned placentae appear to suffer from failure of histone modification-based reprogramming in these (and potentially other) developmentally important genes, leading to aberrant expression of their protein products. Expand
Epigenetic characterization of the PBEF and TIMP-2 genes in the developing placentae of normal mice.
It is suggested that epigenetic changes may regulate gene expression level in the developing placentae of normal mice and that inappropriate epigenetic reprogramming might be one cause of the abnormal placenta seen in cloned animals. Expand
Transcriptome profile of bovine elongated conceptus obtained from SCNT and IVP pregnancies
In the present study we analyzed the gene expression changes induced by somatic cell nuclear transfer (SCNT) and in vitro production (IVP) in bovine elongated embryos using Affymetrix bovine genomeExpand
Involvement of ubiquitin–proteasome system in icariin‐induced cardiomyocyte differentiation of embryonic stem cells using two‐dimensional gel electrophoresis
Results indicated that UPS played an important role in ES cell differentiate into cardiomyocytes induced by icariin, and suggested UPS could be a means by which icARIin may regulate expressions of key proteins that controlCardiomyocyte differentiation. Expand
Proteomics Analysis of Lungs from Somatic Cell Nuclear Transfer (SCNT) Bama Miniature Pig (Sus scrofa)
With the advent of transgenic pigs using the somatic cell nuclear transfer (SCNT), SCNT pig was thought to hold great promise in basic research, as well as in agricultural and biomedicalExpand
DNA Methylation Errors in Cloned Mouse Sperm by Germ Line Barrier Evasion1
It is suggested that DNA methylation errors resulting from embryo cloning are transmitted to the sperm genome by evading the germ line reprogramming barrier. Expand


Abnormal expression of TIMP-2, SOD, vimentin and PAI proteins in cloned bovine placentae.
Western blot analysis suggests that key proteins of placental development are abnormally expressed in SCNT cloned bovine placentae, probably resulting in abnormal placental function and clonal mortality. Expand
Protein profiles of bovine placenta derived from somatic cell nuclear transfer
A global proteomics approach using 2‐DE and MS revealed composite profiles of key proteins involved in abnormal placenta derived from SCNT, and suggested expression abnormality of these genes in SCNT placentA, resulting in fetal losses following SCNT. Expand
Expression profiling of placentomegaly associated with nuclear transplantation of mouse ES cells.
It is indicated that placentomegaly in ES-derived NT mice is associated with large-scale dysregulation of normal gene expression patterns, and the presence of two regulatory pathways that may lead to histologically discernable placentegaly is suggested. Expand
Expression and function of matrix metalloproteinases and their inhibitors at the maternal-embryonic boundary during mouse embryo implantation.
It is concluded that administration of MMP inhibitors retards decidual remodeling and growth, and it is suggested that the MMPs expressed in precursor stromal cells promote their differentiation and expansion. Expand
Abnormal gene expression in cloned mice derived from embryonic stem cell and cumulus cell nuclei
The authors' results demonstrate frequent abnormal gene expression in clones, in which most expression abnormalities appear common to the NT procedure whereas others appear to reflect the particular donor nucleus. Expand
Alterations of expression of developmentally important genes in preimplantation bovine embryos by in vitro culture conditions: implications for subsequent development.
Messenger RNA phenotyping of genes essential in early development provides a useful tool to assess the normality of the produced embryos and a tool to optimize in vitro culture conditions for bovine embryos. Expand
A novel regulatory region is required for trophoblast-specific transcription in transgenic mice.
An analysis of the regulatory region of a trophoblast-specific gene termed 4311 shows that cloned 4311 upstream sequences are able to confer the correct temporal and spatial expression pattern of 4311 on a reporter gene in transgenic mice. Expand
Placentomegaly in Cloned Mouse Concepti Caused by Expansion of the Spongiotrophoblast Layer1
Interestingly, cloned mouse fetuses appeared to be smaller than those of litter size-matched controls, suggesting that cloned mice fetuses were under a latent negative effect on their growth, probably because the placentas are not fully functional. Expand
The effects of pre-B-cell colony-enhancing factor on the human fetal membranes by microarray analysis.
Pre-B-cell colony-enhancing factor appears to be at the proximal end of the pathway to labor initiation and may link sterile distention- induced labor with that of infection-induced labor. Expand
Different molecular mechanisms underlie placental overgrowth phenotypes caused by interspecies hybridization, cloning, and Esx1 mutation
  • U. Singh, L. Fohn, +12 authors R. Fundele
  • Biology, Medicine
  • Developmental dynamics : an official publication of the American Association of Anatomists
  • 2004
Comparison of gene expression profiles of approximately 13,000 expressed sequence tags (ESTs) identified specific subsets of genes with changed expression levels in IHPD, cloned, and Esx1 mutant placentas that are likely to be important in the occurrence of placentopathy. Expand